ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
If we switch from making use of acetonitrile to tetrahydrofuran, as an example, we find that benzoic acid elutes far more rapidly and that p
a values, the pH from the mobile phase has another effect on Every solute’s retention time, making it possible for us to locate the optimum pH for effecting a whole separation on the 4 solutes.
物質の電気化学的な性質を利用した検出器。pHの変動や酸化還元電位の変動を用いて測定を行う。
A number of other detectors are actually used in HPLC. Measuring a change within the cell stage’s refractive index is analogous to monitoring the cellular stage’s thermal conductivity in gas chromatography. A refractive index detector is nearly universal, responding to Nearly all compounds, but has a relatively very poor detection limit of 0.
シリカゲルの粒子径が小さければ小さいほどピークの分離性は良くなるが、送液に必要なポンプの圧力が高くなる。そのため、ポンプ－インジェクター間、インジェクター－カラム間の配管の耐圧を上げたり、カラム自体を比較的高温の下にさらして溶媒の粘度を下げ、抵抗を小さくする工夫をしている。
No matter whether you should boost the efficiency of your seize stage or intensify your whole downstream course of action, Sartorius provides a portfolio of systems specially created to begin developing an successful downstream system tailor-made to your preferences.
高速液体クロマトグラフィーにおいては各物質は比較的鋭いピークとして検出され、分離（他の物質のピークと明確に分けられる）および検出（鋭いピークにより高い感度が得られる）の能力が従来の液体クロマトグラフィーより良くなる。
The focus of caffeine in beverages is decided by a reversed-stage HPLC separation employing read more a cellular stage of 20% acetonitrile and 80% water, and utilizing a nonpolar C8 column. Benefits for any series of 10-μL injections of caffeine benchmarks are in the subsequent table.
원하는 분석 결과를 얻기 위해서는 컬럼도 충분히 고려하고 선택하는 것이 좋습니다.
. The working cylinder as well as equilibrating cylinder to the pump on the left take solvent from reservoir A and send it into the mixing chamber. The pump on the best moves solvent from reservoir B towards the mixing chamber.
The pressurized liquid is usually a mix of solvents which include drinking water, acetonitrile and/or methanol and is also often called the mobile section.
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Resolution: website Precise injection minimizes band broadening, which can cause overlapping peaks and hinder separation.